Abstract: Aiming at the shortcomings of virtual simulation experiment interaction and scene realism,based on constructivist learning theory and situational cognition theory,combined with the interactive characteristics of stylus,using binocular stereo display technology to construct three-dimensional immersive scene,an immersive virtual simulation resource construction plan was designed. The scheme was verified in the biological field practice virtual simulation experiment teaching project. The results show that the scheme is simple,easy to use,expressive,and interactive. It can improve learning efficiency,stimulate students' interest in learning, and satisfy their feelings as a learning subject.