Abstract: Based on the situation in practical teaching, this paper studies the method for cultivating the students’ innovation ability and practical ability under engineering thinking by building the practical teaching from laboratory, innovative base, to enterprise and constructing a new practical teaching model based on the cooperation between school and enterprise. The school practice teaching plays the leader role and enterprise practice teaching provides the supplement. After implementation in major of computer, the results show that the practice teaching model cultivates the students’ engineering thinking ability, improves their innovation ability and practice ability, and shortens the gap between school person and professional person.