Abstract: In order to enrich the content of the microbiological experiment teaching, the experimental teaching center has explored how to add the experiment “the antibacterial effect of Houttuynia cordata on bacteria”. The effective bacteriostasis components of Houttuynia cordata were extracted by direct grinding, water extraction and organic solvent extraction. Then a series of concentration of Staphylococcus aureus and Escherichia coli was set by McFarland’s turbidimetry. The antibacterial effect of Houttuynia cordata on two kinds of bacteria was observed by filter paper method. The results showed that the best extraction method for the effective components of Houttuynia cordata was organic solvent extraction. The extract of Houttuynia cordata had significant inhibitory effect on these two kinds of bacteria, and its bacteriostasis effect on Staphylococcus aureus was stronger than that of Escherichia coli. Therefore, in order to optimize the teaching content and save the teaching resources, the organic solvent extraction method could be used to study the inhibitory effect of Houttuynia cordata on these two kinds of bacteria in actual teaching.