Abstract: The experimental center laboratory of biology carries a large number of experimental teaching activities. It has some characteristics, including large number of participants, large mobility, weak safety awareness, open sharing, various types of courses. It it easy to cause laboratory safety accidents. In order to ensure the safe and efficient operation of the experimental center laboratory of biology in the the whole year, an implementation path for the closed-loop management of the experimental center laboratory safety of biology was proposed, including hazard management, laboratory safety check, laboratory staffs, laboratory safety education, laboratory bio-safety, hazardous chemicals safety, laboratory waste safety and measures for handling laboratory safety accidents. We can provide standard method guidance for management of biological experiment center safety.