Abstract: In order to break through the difficulties of C language experiment teaching, guided by OBE theory, the teaching innovation of C language programming experiment course was implemented: By innovating the experimental classroom teaching mode, the result orientation of experimental teaching was strengthened, the role of students as the main body of experimental teaching was highlighted, and the operation process of experimental teaching was improved. By the design of guided cases, the interconnection between guided cases and experimental projects, and the application of guided cases in experimental teaching, experimental teaching methods have been reformed and experimental teaching content has been updated. The practice results show that the teaching innovation of C language programming design experiment course has optimized the teaching objectives, strengthened the theoretical support for the teaching innovation of the computer programming design experiment courses, and promoted the teaching reform of experiment courses.